Article
Haptoglobin phenotyping by newly developed monoclonal antibodies. Demonstration of haptoglobin uptake into peripheral blood neutrophils and monocytes.
Journal of immunology (Baltimore, Md. : 1950) - 1 Mar 1996
Wagner L, Gessl A, Parzer S B, Base W, Waldhäusl W, Pasternack M S
Abstract excerpt
We have generated two IgG murine mAbs that recognize native human haptoglobin (Hp). These mAbs, 3A8 and 4B2, efficiently bind to the Hp complex regardless of the serum donor's phenotype. The specificity of mAb 3A8 was confirmed by immunoaffinity purification of 3A8-binding material from human ser...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Biological Transport
- Epitopes
- Exocytosis
- Female
- Haptoglobins
- Humans
- Hybridomas
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Monocytes
- Neutrophils
- Phagocytosis
- Phenotype