Article
Cloning and genetic diversity analysis of a new P5CS gene from common bean (Phaseolus vulgaris L.).
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 May 2010
Chen Jibao, Zhang Xiaoyan, Jing Ruilian, Blair Matthew W, Mao Xinguo, Wang Shumin
Abstract excerpt
Delta(1)-pyrroline-5-carboxylate synthetase (P5CS) is the rate-limiting enzyme involved in the biosynthesis of proline in plants. By the 3' rapid amplification of cDNA ends (3'-RACE) approach, a 2,246-bp cDNA sequence was obtained from common bean (Phaseolus vulgaris L.), denominated PvP5CS2 differing from another P5CS gene that we cloned previously from common bean (PvP5CS). The predicted amino acid sequence of...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Chromosome Mapping
- Cloning, Molecular
- Evolution, Molecular
- Genes, Plant
- Genetic Markers
- Genetic Variation
- Linkage Disequilibrium
- Molecular Sequence Data
- Phaseolus
- Phosphotransferases (Alcohol Group Acceptor)