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Article

Mutational analysis of the RNase-like domain in subunit 2 of fission yeast RNA polymerase II.

Molecular & general genetics : MGG - 15 Jan 1996

Kawagishi-Kobayashi M, Yamamoto M, Ishihama A

Abstract excerpt

Local sequence similarity exists between the subunit 2 of eukaryotic RNA polymerases II and the barnase-type bacterial RNases. The RNase-like domain from the Rpb2 of Schizosaccharomyces pombe was expressed in Escherichia coli as a GST fusion protein and examined for its RNase activity. When the GST fusion protein was incubated in vitro with 32P-labeled RNA, the RNA degradation activity was less than 0.1%, if any,...

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PMID: 8569679
DOI: 10.1007/BF02191819

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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