Article
Construction and characterization of Bordetella pertussis RecA- mutants.
FEMS microbiology letters - 1 Nov 1995
Robison C S, Kuhl S A
Abstract excerpt
Antibiotic drug-resistance cassettes (DRCs) were used to insertionally inactivate the wild-type Bordetella pertussis recA gene cloned into a suicide vector. The mutant allele was mobilized by conjugal gene transfer from Escherichia coli strain SM10 into different genetic backgrounds of B. pertussis. Southern hybridization studies of one of these mutants showed that it contained a DRC integrated within a recA gene...
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