Construction and characterization of Bordetella pertussis RecA- mutants.

doi:10.1016/0378-1097(95)00325-y

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Article

Construction and characterization of Bordetella pertussis RecA- mutants.

FEMS microbiology letters - 1 Nov 1995

Robison C S, Kuhl S A

Abstract excerpt

Antibiotic drug-resistance cassettes (DRCs) were used to insertionally inactivate the wild-type Bordetella pertussis recA gene cloned into a suicide vector. The mutant allele was mobilized by conjugal gene transfer from Escherichia coli strain SM10 into different genetic backgrounds of B. pertuss...

Topics

Bacterial Capsules
Bordetella pertussis
DNA Repair
DNA, Bacterial
Glycoproteins
Hemolysis
Methyl Methanesulfonate
Mutagenesis, Insertional
Mutation
Rec A Recombinases
Recombination, Genetic
Ultraviolet Rays
Virulence

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