Article
Cloning and sequence analysis of the pfl gene encoding pyruvate formate-lyase from Streptococcus mutans.
Infection and immunity - 1 Feb 1996
Yamamoto Y, Sato Y, Takahashi-Abbe S, Abbe K, Yamada T, Kizaki H
Abstract excerpt
We have isolated a sorbitol-negative mutant of Streptococcus mutans GS-5 following random mutagenesis with plasmid pVA891 clone banks. This mutant did not metabolize sorbitol anaerobically but did so aerobically. A 10-kb chromosomal DNA fragment flanking the pVA891 insertion was deleted in this mutant. The corresponding region from the parental strain GS-5 was then recovered by a marker rescue method with...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
