Cloning and sequence analysis of the pfl gene encoding pyruvate formate-lyase from Streptococcus mutans.

doi:10.1128/iai.64.2.385-391.1996

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Article

Cloning and sequence analysis of the pfl gene encoding pyruvate formate-lyase from Streptococcus mutans.

Infection and immunity - 1 Feb 1996

Yamamoto Y, Sato Y, Takahashi-Abbe S, Abbe K, Yamada T, Kizaki H

Abstract excerpt

We have isolated a sorbitol-negative mutant of Streptococcus mutans GS-5 following random mutagenesis with plasmid pVA891 clone banks. This mutant did not metabolize sorbitol anaerobically but did so aerobically. A 10-kb chromosomal DNA fragment flanking the pVA891 insertion was deleted in this m...

Topics

Acetyltransferases
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Genes, Bacterial
Molecular Sequence Data
Mutation
Open Reading Frames
Streptococcus mutans

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