Article
PCR haplotypes for the human Y chromosome based on alphoid satellite DNA variants and heteroduplex analysis.
Gene - 20 Nov 1995
Santos F R, Pena S D, Tyler-Smith C
Abstract excerpt
We have developed a system for revealing informative and useful haplotypes for the human Y chromosome using PCR. Variant alphoid satellite DNA subunits were amplified and analysed by digestion with HindIII to score a restriction site polymorphism, or on polyacrylamide gels to reveal 13 heteroduplex haplotypes. Heteroduplexes are double-stranded DNA molecules containing mismatches; the haplotype is the combination...
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