Article
In Azotobacter vinelandii hydrogenase, substitution of serine for the cysteine residues at positions 62, 65, 294, and 297 in the small (HoxK) subunit affects H2 oxidation [corrected].
Journal of bacteriology - 1 Jun 1993
Sayavedra-Soto L A, Arp D J
Abstract excerpt
The essential role of the small (HoxK) subunit of hydrogenase of Azotobacter vinelandii in H2 oxidation was established. This was achieved by modification of the two Cys-X2-Cys amino acid motifs at the N and C termini of the HoxK subunit (Cys-62, -65, -294, and -297). The Cys codons were individu...
Topics
- Amino Acid Sequence
- Azotobacter vinelandii
- Benzyl Viologen
- Cysteine
- Deuterium
- Electron Transport
- Hydrogen
- Hydrogenase
- Methylene Blue
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oxidation-Reduction
- Oxygen
- Phenotype