Article
The identification, characterization, sequencing and mutagenesis of the genes (hupSL) encoding the small and large subunits of the H2-uptake hydrogenase of Azotobacter chroococcum.
Molecular microbiology - 1 Jun 1990
Ford C M, Garg N, Garg R P, Tibelius K H, Yates M G, Arp D J, Seefeldt L C
Abstract excerpt
The structural genes (hupSL) of the membrane-bound NiFe-containing H2-uptake hydrogenase (Hup) of Azotobacter chroococcum were identified by oligonucleotide screening and sequenced. The small subunit gene (hupS) encodes a signal sequence of 34 amino acids followed by a 310-amino-acid, 34156D prot...
Topics
- Amino Acid Sequence
- Azotobacter
- Base Sequence
- Biological Evolution
- DNA, Bacterial
- Genes, Bacterial
- Hydrogen
- Hydrogenase
- Molecular Sequence Data
- Mutation
- Nitrogen Fixation
- Oxidoreductases
- Sequence Homology, Nucleic Acid