Article
Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I. Changes in [4Fe-4S] cluster reduction potential and reactivity.
The Journal of biological chemistry - 15 Nov 1991
Iismaa S E, Vázquez A E, Jensen G M, Stephens P J, Butt J N, Armstrong F A, Burgess B K
Abstract excerpt
We have used site-directed mutagenesis to obtain two variants of Azotobacter vinelandii ferredoxin I (AvFdI), whose x-ray structures are now available. In the C20A protein, a ligand to the [4Fe-4S] cluster was removed whereas in the C24A mutant a free cysteine next to that cluster was removed. Li...
Topics
- Amino Acid Sequence
- Azotobacter vinelandii
- Binding Sites
- Circular Dichroism
- Electron Spin Resonance Spectroscopy
- Ferredoxins
- Genes, Bacterial
- Genotype
- Macromolecular Substances
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oxidation-Reduction
- Protein Conformation
- Sequence Homology, Nucleic Acid