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Article

Analysis of haemoglobin variants using immobilized pH gradients.

European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies - 1 Feb 1993

Sinha P, Galacteros F, Righetti P G, Kohlmeier M, Köttgen E

Abstract excerpt

We present a new method for analysis of haemoglobin variants in immobilized pH gradients. The isoelectric fractionation is performed in polyacrylamide gels with copolymerized pH gradients between pH 7.0 and 8.0. The common haemoglobin variants (HbA, HbF, HbS, HbC, HbE and HbA2) are clearly resolv...

Topics

Evaluation Studies as Topic
Genetic Variation
Hemoglobinopathies
Hemoglobins
Hemoglobins, Abnormal
Humans
Hydrogen-Ion Concentration
Isoelectric Focusing
Reproducibility of Results

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 8467014
DOI: 10.1515/cclm.1993.31.2.91

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8467014-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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