Article
Analysis of haemoglobin variants using immobilized pH gradients.
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies - 1 Feb 1993
Sinha P, Galacteros F, Righetti P G, Kohlmeier M, Köttgen E
Abstract excerpt
We present a new method for analysis of haemoglobin variants in immobilized pH gradients. The isoelectric fractionation is performed in polyacrylamide gels with copolymerized pH gradients between pH 7.0 and 8.0. The common haemoglobin variants (HbA, HbF, HbS, HbC, HbE and HbA2) are clearly resolved within this pH range. The high resolving power and reproducibility of immobilized pH gradients combined with...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
