Article
Variants of Shiga-like toxin II constitute a major toxin component in Escherichia coli O157 strains from patients with haemolytic uraemic syndrome.
Journal of medical microbiology - 1 May 1994
Rüssmann H, Schmidt H, Heesemann J, Caprioli A, Karch H
Abstract excerpt
The prevalence and genotype of Shiga-like toxins (SLTs) in Escherichia coli (O)157 strains from patients in Germany with haemolytic uraemic syndrome (HUS) were investigated. This was done by PCR amplification of the B-subunit genes with two primer pairs--one complementary to slt-IB, and the other...
Topics
- Animals
- Bacterial Toxins
- Base Sequence
- Blotting, Southern
- Child
- DNA Primers
- Enterotoxins
- Enzyme-Linked Immunosorbent Assay
- Escherichia coli
- Escherichia coli Infections
- Gene Expression Regulation, Bacterial
- Genotype
- Hemolytic-Uremic Syndrome
- Humans
- Molecular Sequence Data
- Phenotype
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length