Article
The E. coli envY gene encodes a high affinity opioid binding site.
Neurochemical research - 1 Jul 1993
Cabon F, Morser J, Parmantier E, Solly S K, Pham-Dinh D, Zalc B
Abstract excerpt
In an attempt to isolate a cDNA encoding an opioid receptor, a cDNA library was constructed in the lambda ZAP vector using NG108-15 mRNA as template. Using an in vitro transcription-translation assay and a sib selection strategy, a single phage was isolated. An RNA transcribed from this cDNA was able to direct in vitro translation of opioid binding sites. The insert was sequenced and comparison with data banks...
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