Article
Covalent catalysis in nucleotidyl transfer. A KTDG motif essential for enzyme-GMP complex formation by mRNA capping enzyme is conserved at the active sites of RNA and DNA ligases.
The Journal of biological chemistry - 5 Apr 1993
Cong P, Shuman S
Abstract excerpt
Vaccinia virus RNA capping enzyme, a heterodimer of 95- and 31-kDa subunits, catalyzes transfer of GMP from GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-guanylate intermediate. The GMP residue is attached to the 95-kDa subunit through a phosphoamide bond to the epsilon-amino gr...
Topics
- Amino Acid Sequence
- Binding Sites
- Catalysis
- Cloning, Molecular
- Conserved Sequence
- DNA Ligases
- Electrophoresis, Polyacrylamide Gel
- Guanosine Monophosphate
- Guanosine Triphosphate
- Lysine
- Methyltransferases
- Molecular Sequence Data
- Multienzyme Complexes
- Mutation
- Nucleotides
- Nucleotidyltransferases
- Phosphoric Monoester Hydrolases
- RNA Ligase (ATP)