Article
Assembly and function of a quaternary signal transduction complex monitored by surface plasmon resonance.
Nature - 23 Sept 1993
Schuster S C, Swanson R V, Alex L A, Bourret R B, Simon M I
Abstract excerpt
We have used surface plasmon resonance biosensor technology to monitor the assembly and dynamics of a signal transduction complex which controls chemotaxis in Escherichia coli. A quaternary complex formed which consisted of the response regulator CheY, the histidine protein kinase CheA, a coupling protein CheW and a membrane-bound chemoreceptor Tar. Using various experimental conditions and mutant proteins, we...
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