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Article

Isolation and Characterization of a Repetitive DNA Sequence from Leishmania infantum: Development of a Visceral Leishmaniasis Polymerase Chain Reaction

1 Sept 1993

Abstract excerpt

To construct a DNA probe specific for protozoa that cause visceral leishmaniasis, we cloned Pst I fragments of Leishmania infantum genomic DNA into a Bluescript II SK vector. A clone of 4.3 kb that contained a highly repetitive sequence was isolated and cut with three restriction enzymes: Hae III, Rsa I, and Sau 3A. After a new molecular cloning step, we isolated and sequenced a 140-basepair (bp) fragment. Two...

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Identifiers

PMID: 8372958
DOI: 10.4269/ajtmh.1993.49.364

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