Molecular Diagnosis of Old World Cutaneous Leishmaniasis and Species Identification by Use of a Reverse Line Blot Hybridization Assay

Abstract excerpt

Reverse line blot hybridization assays (RLB) have been used for the rapid diagnosis and genotyping of many pathogens. The leishmaniases are caused by a large number of species, and rapid, accurate parasite characterization is important in deciding on appropriate therapy. Fourteen oligonucleotide probes, 2 genus specific and 12 species specific (2 specific for Leishmania major, 3 for L. tropica, 1 for L. infantum,...