Article
RAPD method useful for distinguishing Leishmania species: design of specific primers for L. braziliensis.
Parasitology - 1 Dec 2003
Martinez E, Alonso V, Quispe A, Thomas M C, Alonso R, Piñero J E, González A C, Ortega A, Valladares B
Abstract excerpt
The technique of Random Amplification Polymorphic DNA allows fragments of the genome to be amplified by means of polymerase chain reaction (PCR) without previous knowledge of their sequences. The protozoa of the genus Leishmania present great genetic variability, making it difficult to characterize the different species. A method is developed with a single 10-mers long primer, which allows the species L....
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