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Diagnosis of Leishmania Using the Polymerase Chain Reaction: a Simplified Procedure for Field Work

1 Sept 1993

Abstract excerpt

Oligonucleotide primers directed to the minicircle kinetoplast DNA of Leishmania strains supported enzymatic amplification of this DNA by the polymerase chain reaction (PCR). A single product of 70 basepairs was obtained from parasites belonging exclusively to the L. braziliensis complex. Direct sequencing of the amplified product confirmed its minicircle origin. Skin biopsy specimens from human patients were...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 8396860
DOI: 10.4269/ajtmh.1993.49.348

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