Article
Rapid production of full-length, infectious geminivirus clones by abutting primer PCR (AbP-PCR).
Journal of virological methods - 1 Jun 1993
Briddon R W, Prescott A G, Lunness P, Chamberlin L C, Markham P G
Abstract excerpt
The application of the polymerase chain reaction (PCR) method of DNA amplification for the isolation of full-length, infectious clones of geminiviruses is described. Non-overlapping, abutting 20-mer oligonucleotide primers were used to produce a linear product from the circular geminivirus genomic template. Clones of African cassava mosaic virus (ACMV) DNA A, obtained by this method, were infectious following...
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