Article
Mutational analysis of complementary-sense genes of African cassava mosaic virus DNA A.
The Journal of general virology - 1 May 1991
Etessami P, Saunders K, Watts J, Stanley J
Abstract excerpt
We have investigated the ability of African cassava mosaic virus DNA A mutants, containing disrupted complementary-sense genes, to infect Nicotiana benthamiana and to replicate in Nicotiana tabacum protoplasts. Three overlapping open reading frames (ORFs) with the capacity to encode proteins with an Mr greater than 10K (AC1, AC2 and AC3) are highly conserved between geminiviruses that infect dicotyledonous plants...
Topics
- DNA Mutational Analysis
- DNA Probes
- DNA, Viral
- Electric Conductivity
- Genes, Viral
- Genetic Complementation Test
- Manihot
- Mosaic Viruses
- Mutagenesis, Site-Directed
- Mutation
- Open Reading Frames
