Article
Polymerase Chain Reaction for the Diagnosis of Candidemia
1 Sept 1993
Abstract excerpt
A PCR assay was developed for the diagnosis of candidemia. Primers were selected to amplify a 158-bp segment from the Candida actin gene that was detected by hybridization with a 32P-labeled oligomer probe. The lower limit of detection was DNA extracted from 10 Candida yeasts/sample, or 25 fg of purified Candida albicans DNA. This PCR was specific for medically important Candida species. Circulating Candida DNA...
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