Close linkage in Pseudomonas stutzeri of the structural genes for respiratory nitrite reductase and nitrous oxide reductase, and other essential genes for denitrification.

Abstract excerpt

The structural gene, nirS, for the respiratory nitrite reductase (cytochrome cd1) from Pseudomonas stutzeri was identified by (i) sequencing of the N-terminus of the purified protein and partial sequencing of the cloned gene, (ii) immunoscreening of clones from a lambda gt11 expression library, (iii) mapping of the transposon Tn5 insertion site in the nirS mutant strain MK202, and (iv) complementation of strain...

Topics

• Amino Acid Sequence
• Genes, Bacterial
• Genetic Complementation Test
• Genetic Linkage
• Immunoblotting
• Molecular Sequence Data
• Nitrite Reductases
• Nitrites
• Oxidoreductases
• Phenotype