Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Recombinant human butyrylcholinesterase G390V, the fluoride-2 variant, expressed in Chinese hamster ovary cells, is a low affinity variant.

The Journal of biological chemistry - 5 Jul 1993

Masson P, Adkins S, Gouet P, Lockridge O

Abstract excerpt

Kinetics of recombinant fluoride-2 variant of human butyrylcholinesterase (Gly390 Val) secreted by Chinese hamster ovary cells were compared to recombinant usual and to usual butyrylcholinesterase purified from human plasma. The usual and fluoride-2 variant were indistinguishable with regard to hydrolysis of benzoylcholine (Km = 5 microM), neutral esters, and at high concentrations of acetylthiocholine,...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Sequence
Share to Base Sequence

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Binding Sites

Share to Cloning, Molecular

Share to Cricetinae

Share to Genetic Variation

Share to Kinetics

Share to Molecular Sequence Data

Source

Identifiers

PMID: 8314794

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Recombinant human butyrylcholinesterase G390V, the fluoride-2 variant, expressed in Chinese hamster ovary cells, is a low affinity variant.PMID 8314794Read ↗

Catalytic parameters for the hydrolysis of butyrylthiocholine by human serum butyrylcholinesterase variants.Semantic proximity Read ↗

Identification of two different point mutations associated with the fluoride-resistant phenotype for human butyrylcholinesterase.Semantic proximity Read ↗

Phenotypic and molecular biological analysis of human butyrylcholinesterase variants.Semantic proximity Read ↗

Human butyrylcholinesterase L330I mutation belongs to a fluoride-resistant gene, by expression in human fetal kidney cells.Semantic proximity Read ↗

Interaction of human butyrylcholinesterase variants with bambuterol and terbutaline.Semantic proximity Read ↗

Role of aspartate 70 and tryptophan 82 in binding of succinyldithiocholine to human butyrylcholinesterase.Semantic proximity Read ↗

Site-directed mutagenesis of active site residues reveals plasticity of human butyrylcholinesterase in substrate and inhibitor interactions.Semantic proximity Read ↗

Activity and polymorphisms of butyrylcholinesterase in a Polish population.Semantic proximity Read ↗

Characterization of an unstable variant (BChE115D) of human butyrylcholinesterase.Semantic proximity Read ↗

An improvement in segregation of human butyrylcholinesterase phenotypes having the fluoride-resistant variants.Semantic proximity Read ↗

An improved cocaine hydrolase: the A328Y mutant of human butyrylcholinesterase is 4-fold more efficient.Semantic proximity Read ↗

Identification of human plasma cholinesterase variants in 6,688 individuals using biochemical analysis.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.