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Article

Effects of Cryopreservation Procedures on the Cytology and Fertilization Rate of In Vitro-Matured Bovine Oocytes1

1 Jul 1999

Abstract excerpt

The survival and developmental capacity of bovine oocytes after cryopreservation are greatly impaired, possibly due to organelle damage caused by freezing procedures. Distributions of chromosomes, microtubules, and microfilaments in bovine oocytes matured in vitro were examined after cooling, ethylene glycol (EG) exposure, or freezing. Oocytes were incubated after treatment for 20 min or 1 or 3 h, fixed, and...

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PMID: 10377047
DOI: 10.1095/biolreprod61.1.178

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