Article
Effects of cooling germinal vesicle-stage bovine oocytes on meiotic spindle formation following in vitro maturation
1 Dec 1999
Abstract excerpt
Attempts to cryopreserve bovine oocytes result in low survival because of their sensitivity to temperatures near 0 degrees C. This study evaluates the effects of chilling germinal vesicle-stage (GV) oocytes on their formation of microtubules and the meiotic spindle. In experiment 1, five groups of GV-stage oocytes, each consisting of approximately 90 oocytes, were held at 39 degrees C as controls, or at 31...
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