Article
Mutagenic, electrochemical, and crystallographic investigation of the cytochrome b5 oxidation-reduction equilibrium: involvement of asparagine-57, serine-64, and heme propionate-7.
Biochemistry - 12 Jun 1990
Funk W D, Lo T P, Mauk M R, Brayer G D, MacGillivray R T, Mauk A G
Abstract excerpt
A gene coding for lipase-solubilized bovine liver microsomal cytochrome b5 has been synthesized, expressed in Escherichia coli, and mutated at functionally critical residues. Characterization of the recombinant protein revealed that it has a reduction potential that is approximately 17 mV lower t...
Topics
- Amino Acid Sequence
- Animals
- Asparagine
- Base Sequence
- Cattle
- Cytochromes b5
- DNA
- Electrochemistry
- Escherichia coli
- Heme
- Molecular Sequence Data
- Mutation
- Oxidation-Reduction
- Protein Conformation
- Serine
- X-Ray Diffraction