Article
The ogd1 and kgd1 mutants lacking 2-oxoglutarate dehydrogenase activity in yeast are allelic and can be differentiated by the cloned amber suppressor.
Current genetics - 1 Nov 1993
Mockovciaková D, Janitorová V, Zigová M, Kaclíková E, Zagulski M, Subík J
Abstract excerpt
The activity of mitochondrial 2-oxoglutarate dehydrogenase in S. cerevisiae can be impaired either by the ogd1 or the kgd1 mutation. The OGD1 gene and two suppressor genes were isolated by complementation of the ogd1 mutant. The complementation of the kdg1 mutant by the OGD1 gene, an allelism tes...
Topics
- Alleles
- Base Sequence
- Cloning, Molecular
- DNA, Fungal
- Genes, Fungal
- Genetic Complementation Test
- Ketoglutarate Dehydrogenase Complex
- Molecular Sequence Data
- RNA, Fungal
- RNA, Transfer, Gln
- Restriction Mapping
- Saccharomyces cerevisiae
- Suppression, Genetic