Article
X-ray structure of two complexes of the Y143F flavocytochrome b2 mutant crystallized in the presence of lactate or phenyl lactate.
Biochemistry - 8 Aug 1995
Tegoni M, Begotti S, Cambillau C
Abstract excerpt
Flavocytochrome b2 is a flavohemo enzyme localized in the intermembrane space of yeast mitochondria, where it catalyzes the electron transfer from its substrate, L-lactate, to cytochrome c. We have obtained crystals of a flavocytochrome b2 mutant, Y143F, which are isostructural with those of the...
Topics
- Binding Sites
- Crystallography, X-Ray
- Flavoproteins
- L-Lactate Dehydrogenase
- L-Lactate Dehydrogenase (Cytochrome)
- Lactates
- Lactic Acid
- Models, Molecular
- Mutation
- Oxidation-Reduction
- Phenylpyruvic Acids
- Protein Conformation