Article
Probing actin incorporation into myofibrils using Asp11 and His73 actin mutants.
Cell motility and the cytoskeleton - 1 Jan 1993
Xia D, Peng B, Sesok D A, Peng I
Abstract excerpt
We used a cell free system Bouché et al.: J. Cell Biol. 107:587-596, 1988] to study the incorporation of actin into myofibrils. We used alpha-skeletal muscle actin and actins with substitutions of either His73 [Solomon and Rubenstein: J. Biol.Chem. 262:11382, 1987], or Asp11 [Solomon et al.: J. Biol. Chem. 263:19662, 1988]. Actins were translated in reticulocyte lysate and incubated with myofibrils. The...
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