Article
Overproduction, purification and characterization of the Escherichia coli ferritin.
European journal of biochemistry - 15 Dec 1993
Hudson A J, Andrews S C, Hawkins C, Williams J M, Izuhara M, Meldrum F C, Mann S, Harrison P M, Guest J R
Abstract excerpt
Recent studies have indicated that Escherichia coli possesses at least two iron-storage proteins, the haem-containing bacterioferritin and ferritin. The ferritin protein has been amplified 600-fold to 11-14% of total cell protein in a bfr mutant and purified to homogeneity with an overall yield of 13%. The cellular ferritin content remained relatively constant throughout the growth cycle and amplification was...
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