Article
Human corneal aldehyde dehydrogenase: purification, kinetic characterisation and phenotypic variation.
Biochemistry and molecular biology international - 1 Sept 1993
King G, Holmes R S
Abstract excerpt
Human corneal aldehyde dehydrogenase (designated ALDH3) was purified to homogeneity and characterised with respect to substrate specificity and inhibition by thiol reagents. The enzyme was present as a major soluble protein (5% of the total soluble protein) and was found to efficiently catalyse t...
Topics
- Aldehyde Dehydrogenase
- Animals
- Cattle
- Cornea
- Disulfiram
- Electrophoresis, Polyacrylamide Gel
- Humans
- Isoelectric Focusing
- Oxidation-Reduction
- Phenotype
- Spectrophotometry, Ultraviolet
- Substrate Specificity
