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Article

The concentrations of glutamine and ammonia in commercially available cell culture media

1 Nov 1993

Abstract excerpt

The amino acid glutamine is an essential nutrient for cells in culture. In aqueous solutions such as liquid culture media, glutamine spontaneously decomposes into ammonia. In this study, we examined the toxicity of ammonia for two different cell lines. In mouse hybridoma cell cultures, viable cell counts were reduced at exogenous ammonia concentrations of 1000 microM. In the human promyelocytic cell line however,...

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PMID: 8228290
DOI: 10.1016/0022-1759(93)90331-z

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