The use of chemiluminescence and the ELISA spot assay to identify and enumerate rare immunoglobulin switch variants.

Abstract excerpt

The use of chemiluminescence and the ELISA spot assay for identifying rare immunoglobulin switch variants is described. The technique utilizes nitrocellulose membranes and allows rapid screening of a large number of cells. The number of spots can be recorded either manually or automatically by using a commercially available colony counting program. This modification of the ELISA spot assay makes it less labor...

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