Simultaneous expression of kappa and lambda light chains in a murine IgG3 anti-Cryptococcus neoformans hybridoma cell line.

doi:10.1089/hyb.1994.13.531

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Simultaneous expression of kappa and lambda light chains in a murine IgG3 anti-Cryptococcus neoformans hybridoma cell line.

Hybridoma - 1 Dec 1994

Spira G, Yuan R, Paizi M, Weisendal M, Casadevall A

Abstract excerpt

Allelic exclusion normally results in the expression of only one light and one heavy chain gene. However, some hybridomas have been reported to express two different heavy chain genes. Here we report that the IgG3 hybridoma 4H3.C8B expresses both kappa and lambda light chains. 4H3.C8B was originally recovered by screening for antibody binding to Cryptococcus neoformans polysaccharide antigen and characterized as...

Topics

Alleles
Animals
Antibodies, Fungal
Antigens, Fungal
Cryptococcus neoformans
Enzyme-Linked Immunosorbent Assay
Gene Expression Regulation
Genes, Immunoglobulin
Hybridomas
Immunoglobulin Class Switching

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Immunoglobulin G

Immunoglobulin kappa-Chains

Immunoglobulin lambda-Chains

Mice

Mice, Inbred BALB C

Mice, Inbred NZB

Polysaccharides

Source

Open in PubMed

Identifiers

PMID: 7737677
DOI: 10.1089/hyb.1994.13.531

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7737677-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 8 closest

Simultaneous expression of kappa and lambda light chains in a murine IgG3 anti-Cryptococcus neoformans hybridoma cell line.PMID 7737677Read ↗

Use of mutagens to increase rate of immunoglobulin isotype switching of hybridoma cells.Semantic proximity Read ↗

Identification of rare immunoglobulin switch variants using the ELISA spot assay.Semantic proximity Read ↗

Immunoglobulin heavy chain constant region determines the pathogenicity and the antigen-binding activity of rheumatoid factor.Semantic proximity Read ↗

The use of chemiluminescence and the ELISA spot assay to identify and enumerate rare immunoglobulin switch variants.Semantic proximity Read ↗

Molecular characterization of the humoral responses to Cryptococcus neoformans infection and glucuronoxylomannan-tetanus toxoid conjugate immunization.Semantic proximity Read ↗

IL-4 plus CD40 monoclonal antibody induces human B cells γ subclass-specific isotype switch: switching to γ 1, γ 3, and γ 4, but not γ 2Semantic proximity Read ↗

Ig heavy chain expression and class switching in vitro from an allele lacking the 3' enhancers DNase I-hypersensitive hs3A and hs1,2.Semantic proximity Read ↗

Immune response in the hamster: definition of a novel IgG not expressed in all hamster strains.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.