Article
Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae.
FEBS letters - 25 Oct 1993
Tsukada M, Ohsumi Y
Abstract excerpt
Autophagy in the yeast is similar to that in mammalian cells. A mutant designated as apg1 (autophagy) defective in accumulation of autophagic bodies in the vacuoles was isolated by selection using a light microscope from a mutagenized proteinase-deficient strain. In the apg1 strain, which has normal vacuolar proteinases, nitrogen starvation did not induce protein degradation. The apg1 mutant lost its viability...
Topics
- Autophagy
- Genetic Complementation Test
- Kinetics
- Mutation
- Organelles
- Phenotype
- Saccharomyces cerevisiae
- Vacuoles