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Article

A newly identified MHV-A59 ORF1a polypeptide p65 is temperature sensitive in two RNA negative mutants.

Advances in experimental medicine and biology - 1 Jan 1993

Hughes S A, Denison M R, Bonilla P, Leibowitz J L, Baric R S, Weiss S R

Abstract excerpt

Polypeptide products of MHV-A59 gene 1 have been identified in infected DBT cells and in the products of in vitro translations of genome RNA. In this paper we report the identification in infected cell lysates of a 65-kDa polypeptide (p65) encoded in ORF 1a. Studies on the kinetics of appearance and processing of p65 show that p65 is detectable after p28 but before the appearance of p290, p240 and p50. No...

Topics

Animals
Astrocytoma
Mice
Murine hepatitis virus
Mutation
Open Reading Frames
Protein Biosynthesis
Protein Processing, Post-Translational
RNA, Viral
Temperature
Tumor Cells, Cultured

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Viral Proteins

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Identifiers

PMID: 8209734
DOI: 10.1007/978-1-4615-2996-5_35

Genes

DBT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8209734-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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