Article
Comparative roles of histidine 51 in human beta 1 beta 1 and threonine 51 in pi pi alcohol dehydrogenases.
Archives of biochemistry and biophysics - 1 Jun 1994
Davis G J, Carr L G, Hurley T D, Li T K, Bosron W F
Abstract excerpt
Histidine at position 51 of the class I beta 1 alcohol dehydrogenase (ADH) functions as a general base by indirectly abstracting a proton from the alcohol substrate through a hydrogen-bonded proton relay system. The human class II pi-ADH was reported to be polymorphic, having either Ser or Thr, b...
Topics
- Alcohol Dehydrogenase
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Cloning, Molecular
- DNA Primers
- DNA, Complementary
- Genetic Variation
- Genotype
- Histidine
- Humans
- Hydrogen Bonding
- Isoenzymes
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Polymerase Chain Reaction
- Recombinant Proteins