Heteroduplex molecules formed between allelic sequences cause nonparental RAPD bands.

doi:10.1093/nar/22.9.1632

Back to search

Article

Heteroduplex molecules formed between allelic sequences cause nonparental RAPD bands.

Nucleic acids research - 11 May 1994

Ayliffe M A, Lawrence G J, Ellis J G, Pryor A J

Abstract excerpt

Primers (10-mers) of random sequence were used to amplify RAPD bands from genomic DNA of an F1 strain of flax rust (Melampsora lini) and its two parent strains. One primer out of 160 tested was unusual in that it amplified a product from F1 DNA that was not amplified from either parental DNAs. Th...

Topics

Alleles
Base Sequence
Basidiomycota
DNA
Molecular Sequence Data
Nucleic Acid Heteroduplexes
Polymerase Chain Reaction
Polymorphism, Genetic

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.