Article
Heteroduplex molecules formed between allelic sequences cause nonparental RAPD bands.
Nucleic acids research - 11 May 1994
Ayliffe M A, Lawrence G J, Ellis J G, Pryor A J
Abstract excerpt
Primers (10-mers) of random sequence were used to amplify RAPD bands from genomic DNA of an F1 strain of flax rust (Melampsora lini) and its two parent strains. One primer out of 160 tested was unusual in that it amplified a product from F1 DNA that was not amplified from either parental DNAs. The same primer also generated two RAPD bands that segregated as codominant alleles amongst F2 progeny. The nonparental...
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