Article
Predigestion of DNA template improves the level of polymorphism of random amplified polymorphic DNAs in wheat.
Genetic analysis : biomolecular engineering - 1 Mar 1995
Koebner R M
Abstract excerpt
Random amplified polymorphic DNA (RAPD) analysis in wheat has proven to be poor in its levels of both reproducibility and polymorphism. By digesting the template, prior to performing PCR, with frequently cutting restriction enzymes, the level of polymorphism was improved. RAPD profiles from certa...
Topics
- DNA Primers
- DNA Restriction Enzymes
- DNA, Plant
- Genotype
- Plant Leaves
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Templates, Genetic
- Triticum