Analysis of single protoplasts and regenerated plants by PCR and RAPD technology.

doi:10.1007/BF00279433

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Article

Analysis of single protoplasts and regenerated plants by PCR and RAPD technology.

Molecular & general genetics : MGG - 1 Mar 1993

Brown P T, Lange F D, Kranz E, Lörz H

Abstract excerpt

We investigated the use of the polymerase chain reaction (PCR) and the associated random amplification of polymorphic DNA (RAPD) technique in the analysis of DNA and specific genes in plant cells at different stages of regeneration in in vitro cultures. We demonstrate that both procedures can be...

Topics

Base Sequence
Cells, Cultured
DNA
Genetic Variation
Molecular Sequence Data
Plants, Toxic
Polymerase Chain Reaction
Protoplasts
Nicotiana
Triticum

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