Generation of DNA-based markers in specific genome regions by two-primer RAPD reactions.

doi:10.1101/gr.4.6.346

Back to search

Article

Generation of DNA-based markers in specific genome regions by two-primer RAPD reactions.

PCR methods and applications - 1 Jun 1995

Hu J, van Eysden J, Quiros C F

Abstract excerpt

Random amplified polymorphic DNA (RAPD) markers offer quick screening of different regions of the genome for genetic polymorphisms. The standard RAPD procedure uses a single 10-base-long random oligonucleotide as a primer to amplify short stretches of the genome by PCR. We modified the procedure...

Topics

Brassica
DNA Primers
DNA, Plant
Electrophoresis, Agar Gel
Genetic Markers
Genome
Genotype
Polymerase Chain Reaction
Polymorphism, Genetic

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.