Article
Generation of DNA-based markers in specific genome regions by two-primer RAPD reactions.
PCR methods and applications - 1 Jun 1995
Hu J, van Eysden J, Quiros C F
Abstract excerpt
Random amplified polymorphic DNA (RAPD) markers offer quick screening of different regions of the genome for genetic polymorphisms. The standard RAPD procedure uses a single 10-base-long random oligonucleotide as a primer to amplify short stretches of the genome by PCR. We modified the procedure by using two primers in each reaction in a Brassica napus mapping project. We found that the two-primer RAPD tends to...
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