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Article

Histidinol dehydrogenase loses its catalytic function through the mutation of His261-->Asn due to its inability to ligate the essential Zn.

Journal of biochemistry - 1 Jan 1994

Nagai A, Ohta D

Abstract excerpt

Histidinol dehydrogenase (HDH), a Zn-metalloenzyme, produces His from histidinol through two successive oxidation reactions with NAD+ as a coenzyme. A mutation, His261-->Asn, caused the complete loss of the Zn, thereby inactivating the enzyme, without significant structural perturbation. The ability to oxidize an intermediate, histidinaldehyde, was restored to about 4% of that of the wild-type enzyme by adding...

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Identifiers

PMID: 8188630
DOI: 10.1093/oxfordjournals.jbchem.a124298

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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