Histidinol dehydrogenase loses its catalytic function through the mutation of His261-->Asn due to its inability to ligate the essential Zn.

Abstract excerpt

Histidinol dehydrogenase (HDH), a Zn-metalloenzyme, produces His from histidinol through two successive oxidation reactions with NAD+ as a coenzyme. A mutation, His261-->Asn, caused the complete loss of the Zn, thereby inactivating the enzyme, without significant structural perturbation. The ability to oxidize an intermediate, histidinaldehyde, was restored to about 4% of that of the wild-type enzyme by adding...

Topics

• Alcohol Oxidoreductases
• Amino Acid Sequence
• Asparagine
• Base Sequence
• Brassica
• Catalysis
• Histidine
• Molecular Sequence Data
• Mutagenesis, Site-Directed
• Mutation
• NAD