Article
The hypo-osmolarity-sensitive phenotype of the Saccharomyces cerevisiae hpo2 mutant is due to a mutation in PKC1, which regulates expression of beta-glucanase.
Molecular & general genetics : MGG - 1 Mar 1994
Shimizu J, Yoda K, Yamasaki M
Abstract excerpt
To obtain more information about the cell wall organization of Saccharomyces cerevisiae, we have developed a novel screening system to obtain cell wall-defective mutants, using a density gradient centrifugation method. Nine hypo-osmolarity-sensitive mutants were classified into two complementatio...
Topics
- Cell Wall
- Centrifugation, Density Gradient
- Cloning, Molecular
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genetic Complementation Test
- Glucan 1,3-beta-Glucosidase
- Mutation
- Phenotype
- Protein Kinase C
- Saccharomyces cerevisiae
- Temperature
- Water-Electrolyte Balance
- beta-Glucosidase