Article
Characterization of the surfactin synthetase multi-enzyme complex.
Biochimica et biophysica acta - 16 Mar 1994
Galli G, Rodriguez F, Cosmina P, Pratesi C, Nogarotto R, de Ferra F, Grandi G
Abstract excerpt
Three subunits (srfAORF1, srfAORF2 and srfAORF3) of the Bacillus subtilis surfactin synthetase multi-enzyme complex have been identified by SDS-PAGE and Western blot analyses. In accordance with the sequence analysis of the surfactin (srfA) operon, the protein subunits have a molecular mass of 402,000 Da, 401,000 Da and 144,000 Da, respectively. Confirmation of the identity of the proteins was obtained by...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Bacterial Proteins
- Kinetics
- Molecular Sequence Data
- Multienzyme Complexes
- Mutation
- Peptide Synthases