Isolation and characterization of nonsense mutations in gene 10 of bacteriophage phi 6.

Abstract excerpt

Nonsense mutants of bacteriophage phi 6 were isolated by a procedure that involved directed mutagenesis of a cDNA copy of genomic segment M, transcription of this segment, in vitro packaging into procapsids, and transfection of spheroplasts to form viable mutant phage. Recombinant phi 6 viruses that contained amber mutations in two open reading frames, ORF 10 and ORF D, of genomic segment M were isolated. We show...

Topics

• Bacteriophage phi 6
• Base Sequence
• Genes, Viral
• Molecular Sequence Data
• Mutagenesis, Site-Directed
• Mutation
• Nucleic Acid Conformation
• Restriction Mapping
• Viral Proteins
• Viral Structural Proteins
• Virus Replication