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Article

Isolation and characterization of nonsense mutations in gene 10 of bacteriophage phi 6.

Journal of virology - 1 Apr 1994

Johnson M D, Mindich L

Abstract excerpt

Nonsense mutants of bacteriophage phi 6 were isolated by a procedure that involved directed mutagenesis of a cDNA copy of genomic segment M, transcription of this segment, in vitro packaging into procapsids, and transfection of spheroplasts to form viable mutant phage. Recombinant phi 6 viruses that contained amber mutations in two open reading frames, ORF 10 and ORF D, of genomic segment M were isolated. We show...

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Identifiers

PMID: 8139018
DOI: 10.1128/JVI.68.4.2331-2338.1994
PMCID: PMC236709

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