Article
Identification of the cysteine in the steroid-binding site of human corticosteroid binding globulin by site-directed mutagenesis and site-specific chemical modification.
The Journal of steroid biochemistry and molecular biology - 1 Jan 1994
Ghose-Dastidar J, Green R, Ross J B
Abstract excerpt
Binding of corticosteroids by human corticosteroid binding globulin (hCBG) is thought to involve interaction with one of its two cysteine residues (Cys60 and Cys228). To identify which of the two cysteine residues mediates steroid binding, we have produced mutant hCBGs containing serine or alanine in place of Cys228 by site-directed mutagenesis. Alteration of Cys228 to serine or alanine does not change the...
Topics
- Base Sequence
- Binding Sites
- Cysteine
- DNA, Complementary
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Steroids
- Sulfhydryl Reagents
- Transcortin