Article
The relationship between carotenoid biosynthesis and the assembly of the light-harvesting LH2 complex in Rhodobacter sphaeroides.
The Biochemical journal - 15 Feb 1994
Lang H P, Hunter C N
Abstract excerpt
Coloured carotenoids play some undefined role in the assembly of a functional light-harvesting 2 (LH2) complex in photosynthetic bacteria. We have used a series of transposon Tn5 insertion mutants disrupted at various stages of the carotenoid-biosynthetic pathway, together with an LH2 deletion/insertion mutant, to investigate this effect in Rhodobacter sphaeroides. Mutants were initially characterized by...
Topics
- Alkyl and Aryl Transferases
- Blotting, Western
- Carotenoids
- Electrophoresis, Gel, Pulsed-Field
- Electrophoresis, Polyacrylamide Gel
- Genetic Complementation Test
- Geranylgeranyl-Diphosphate Geranylgeranyltransferase
- Mutagenesis, Insertional
- Mutation
- Operon
