Article
Localized transposon Tn5 mutagenesis of the photosynthetic gene cluster of Rhodobacter sphaeroides.
Molecular microbiology - 1 Jun 1990
Coomber S A, Chaudhri M, Connor A, Britton G, Hunter C N
Abstract excerpt
Four genes essential for bacteriochlorophyll biosynthesis were known to be encoded within a 45 kb region of the Rhodobacter sphaeroides genome, the boundaries of which are defined by puh and puf genes for reaction-centre and light-harvesting LH1 complexes. The cluster is represented by eight overlapping inserts cloned in the mobilizable vector pSUP202. We have used localized transposon Tn5 mutagenesis to...
Topics
- Bacteriochlorophylls
- Carotenoids
- Chromosome Mapping
- DNA Transposable Elements
- Light-Harvesting Protein Complexes
- Multigene Family
- Mutation
- Phenotype
- Photosynthesis
- Photosynthetic Reaction Center Complex Proteins