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Article

Purification and characterization of the periplasmic nitrate reductase from Thiosphaera pantotropha.

European journal of biochemistry - 15 Feb 1994

Berks B C, Richardson D J, Robinson C, Reilly A, Aplin R T, Ferguson S J

Abstract excerpt

The periplasmic nitrate reductase of Thiosphaera pantotropha has been purified from a mutant strain (M-6) that overproduces the enzyme activity under anaerobic growth conditions. The enzyme is a complex of a 93-kDa polypeptide and a 16-kDa nitrate-oxidizable cytochrome c552. The complex contains molybdenum; a fluorescent compound with spectral features of a pterin derivative can be extracted. In contrast to the...

Topics

Cell Membrane
Heme
Hydrogen-Ion Concentration
Mass Spectrometry
Membrane Potentials
Molecular Weight
Mutation
Nitrate Reductase
Nitrate Reductases
Oxidation-Reduction
Paracoccus denitrificans

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Identifiers

PMID: 8119278
DOI: 10.1111/j.1432-1033.1994.tb18605.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8119278-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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