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Article

Access to the active site of periplasmic nitrate reductase: insights from site-directed mutagenesis and zinc inhibition studies.

Biochemistry - 28 Aug 2007

Dementin Sébastien, Arnoux Pascal, Frangioni Bettina, Grosse Sandrine, Léger Christophe, Burlat Bénédicte, Guigliarelli Bruno, Sabaty Monique, Pignol David

Abstract excerpt

The periplasmic nitrate reductase (NapAB), a member of the DMSO reductase superfamily, catalyzes the first step of the denitrification process in bacteria. In this heterodimer, a di-heme NapB subunit is associated to the catalytic NapA subunit that binds a [4Fe-4S] cluster and a bis(molybdopterin guanine dinucleotide) cofactor. Here, we report the kinetic characterization of purified mutated heterodimers from...

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PMID: 17676770
DOI: 10.1021/bi700928m

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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